If the semen is preparated for IUI there should be at least 2 x 106 motile spermatozoa per ml of grade a (WHO) or +3 motility. If the semen is prepared for IVF there should be at least 0.1 x 106 motile spermatozoa per ml of grade a (WHO)or +3 motility. For IUI there should be a volume of 0.3-0.5 ml inseminated. Higher concentration of motile spermatozoa will not increase the pregnancy chance significantly, but a lower concentration will result in a dramatically decrease