If spermatozoa is purified by use of a gradient centrifugation or swim-up and afterwards frozen in SpermCryo™ All-round, it can be used directly for IUI or IVF directly after thawing without any further preparation. This means that no wash, centrifugation or other procedures are required. The content of HSA in the freezing media is not harmful in any way. However, there is always a very small risk that the recipient will react to the contents of antibiotics (gentamycin) in the media if the recipient is allergic, but no such cases have been reported yet.